On March 26, 2026, the NeuroConnect Organoid Neurophysiology Workshop was held at the Karlsruhe Institute of Technology (KIT). The workshop brought together over 80 researchers from the fields of neuroscience, bioengineering, medicine, and ethics. Hosted by Cluster Principal Investigators Prof. Dr. Simone Mayer and Prof. Dr. Jasmin Aghassi-Hagmann and organized in collaboration with the University of Freiburg, the event focused on enhancing in vitro models of the human brain. Participants explored advances in engineered neural tissues, including activity-dependent plasticity and integrated electrophysiological recording within organoids. Discussions emphasized the growing convergence of technological innovation and neuroscience, as well as the ethical implications of increasingly complex, brain-like systems. The workshop concluded with a poster session that highlighted emerging ideas and the strong engagement of early-career researchers.